His need to pee was urgent enough to take a break, though 45 seconds isn't enough to evacuate a large bladder. Apparently it wasn't so urgent that he couldn't take a quick swig and presumably resume playing. He's probably played with a full bladder before and figures he can hold it.
You can see at random points during the video he's subtly shaking his legs
When you're going to be talking for over an hour you need to be well hydrated to keep your vocal chords lubricated. (There are tales from actors who have had the same problem.) This is an athletic guy who no doubt has confidence in his ability to hold his bladder, and although the podcast was reaching the end, he was in a hurry to hustle off camera!
Cyclist admits to preferring to "just wet himself" during racing events. He goes through the common options most cyclists choose from when they need to go during a race, discounting each one for their impracticalities before settling on having an accident while you pedal - and the benefits that option brings compared to the others. He also seems to wet himself during the video, to illustrate his point. You can see the wetness on his Lycra suit and towards the end, the camera angle changes to his legs where you can see pee trails down his leg, on his shoes and a couple of drips falling from his crotch.
You'll note he has to do a lot of defending of himself (although to be fair, he just laughs it off) due to the criticism of most of the viewers in the comments section. He's responded to some comments with videos further insisting that pissing yourself is the best option. He's also done a video on soiling himself, if that's your kind of thing. I quite like his determination and what he's prepared to sacrifice to remain focused on winning a race.